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He later left with Ratner and a couple of hot girls.
"He certainly didn''t look like a man with a girlfriend. He was partying in the upstairs VIP area with his best friend, Trace Ayala, Brett Ratner and a group of girls," the New York Post quoted a source as saying.
The source said: "The girls were all over him, fighting to give him their numbers, and he seemed to be enjoying it. There was one model-type blonde he chatted to for a while."
The source added: "At one point, a bodyguard did not recognize Ayala and kept him out of the VIP area, so Timberlake fired him on the spot.
"Around 1:30 a.m., Timberlake left along with Ayala, Ratner and a group of girls, including the model blonde, and headed to the Boom Boom Room."
Timberlake has repeatedly denied rumours that his relationship with Hollywood beauty Biel is on the rocks.