The 'I Like It' singer reportedly lost his temper with Winehouse when she interrupted him during a performance earlier this week.
Iglesias told the BBC that the story was "completely made up".
"The story came from 'a source'. How come they always say 'a source'? I didn't even know Amy Winehouse was there," he claimed.
"The thing that makes it even funnier is I told my manager that I'd do an interview with the paper to show that it was not true, or ask them to retract the story, but it's funny how the media will never retract something. They will never want to prove they're wrong."
The 35-year-old, who is currently promoting his new album Euphoria , expressed his annoyance at the media reporting false information.
"I'm good friends with Ricky Martin's manager and I remember when those pictures came out of him and another guy at the beach, the papers said it was his boyfriend, but it was actually his brother.
"All they had to do was do a little bit of research," he said. "It's funny - you've got to laugh and you can't really take it seriously."
However the singer admitted that he would continue to ignore most press reports.
"I think if they ever said anything that was life-threatening, or if I had a son and the stuff they printed was going to hurt him, then I would take action, but anything stupid I don't care."
