The award, regarded as one of the night's biggest honours, has previously been won by the likes of Halle Berry, Charlize Theron and Cameron Diaz.
"Jennifer Aniston has the magic combination of Hall of Fame hotness and humour that makes every guy wish she lived next door," Spike TV representative Casey Patterson said. "Our audience has been asking for this for years. We're thrilled to be honouring Jennifer."
Aniston rose to fame in her role as Rachel Green on the longrunning sitcom Friends, since which she has carved out a successful career in Hollywood.
Among Aniston's most successful films are Bruce Almighty, Marley & Me and Just Go With It.
Other stars nominated at this year's awards include Natalie Portman, Mila Kunis, Rose Huntington-Whiteley and Minka Kelly.
The 2011 Guys Choice will air June 10 at 9pm ET/PT on Spike TV.
