The couple fought back tears and were clearly overwhelmed by the generosity of the chat-show host, reportsdailymail.co.uk.
Rozonno and Mia McGhee and their sextuplets - Olivia, Madison, Rozonno Jr, Josiah, Elijah and Isaac - appeared on the show after they became an internet sensation when Mia posted a family photo on her Facebook page.
As well as millions of other people all over the world, Oprah saw it and thought: "I love that picture, I have to meet them."
On the show, the couple told the chat-show queen how difficult and expensive it is raising the six babies and revealed they go through 50 diapers and 30 bottles a day.
The couple, who are both from impoverished backgrounds, started dating when they were in high school and married when they were 18 and 19 years old respectively.
They were determined to create the kind of family they never had.
Mia, whose mother was on welfare and whose two brothers were involved in criminal activities, said: "Since I didn't have a father, I've always wanted my children to have a father. I wanted, of course, to graduate from high school, go to college, get married."
While Rozonno runs a carpet-cleaning service, Mia stays at home with the sextuplets, though they said they do get some help during the week.
