The 'Desperate Housewives' actress filed the documents at Los Angeles Superior Court, just hours after denying reports they were planning to split.
"It is with great sadness that after 7 years together, Tony and I have decided to divorce. We love each other deeply and pray for each other's happiness," the Daily Mail quoted her as tweeting.
The news comes after a spokesperson for the 35-year-old actress shot down a report that claimed her basketball player husband had filed for divorce on Monday in Texas.
In the divorce papers, Longoria cites the standard "irreconcilable differences" as the reason for the split. She is also seeking spousal support from Parker.
However, there was no reference to the claims her 28-year-old NBA star husband has been unfaithful.
But Eva confided to her friend Mario Lopez - host of U.S. entertainment show Extra - that Parker had cheated on her during their marriage.
The actress also confirmed to Lopez that she found hundreds of text messages from another woman on Parker''s phone, who turned out to be the wife of one of the NBA player''s teammates at the San Antonio Spurs.
The woman''s identity has not been revealed nor the name of the San Antonio Spurs teammate.
Longoria added that her estranged husband cheated on her with yet another woman with whom he kept in touch via Facebook.
"She is devastated, she wants us all to know that. But she''s strong," Lopez said.
Her husband later tweeted the same message.
Eva is being represented in the divorce by high profile celebrity divorce lawyer Lance Spiegel, who has previously worked for Sandra Bullock in her divorce from Jesse James and Russell Simmons in his divorce from Kimora Lee.
